Enhanced categorization of the transcriptome brings experimenters one-step closer to comprehending the ruling of disease states. An immense deal of focus is put on ribonucleic acids and their interaction with other molecules. The term RNA includes an extensive assortment of molecular sizes, ranging from superior messenger and ribosomal ribonucleic acids to micro and small nucleotide ribonucleic acids, known as miRNAs and snRNAs respectively.
The growing interest
in understanding the authoritarian roles of non-coding small RNAs has encouraged
the need for superior tools during the process of small RNA extract and small
RNA isolation for research studies. Although the kits that are designed for
these processes do not differ in the types of small RNAs, the different
offerings offer diverse levels of RNA isolation and enhancement.
Nowadays, many
companies are offering kits for total RNA isolation and many of them provide kits
specifically for Small RNA isolation,
as well. These kits are commonly known as miRNA kits, although they may contain
other categories of small RNAs, as well. Even though the use of spin columns is
considered convenient for extracting and isolating process is quite common amid
many users, these kits are available in magnetic-bead and multiwell plate formats,
as well. They are also capable of giving
higher throughputs.
Similar to
other types of DNA/RNA isolation kits, there are options of size in terms of
input quantity, with such names as mini or micro kits. These kits include:
·
MirVana miRNA Isolation Kits
·
miRNeasy kits
·
mirPremierTM microRNA Isolation Kits
·
miRNA Purification and Isolation Kits
·
High Pure miRNA Isolation Kits
·
Norgen Biotek's microRNA Purification Kits
Some manufacturers
provide kits for meticulous sample types, such as tissues and cells, plasma or
serum, and FFPE or formalin-fixed paraffin-embedded tissues.
The conventional
approach for the RNA stems isolation from procedures that work well with the
nucleic acid is phenol removal followed by isolation onto a silica filter and the
ethanol precipitation. Phenol takes proteins away from the lysate and facilitates
the withdrawal of all types of nucleic acids. Even though this is the most
reliable method, some tool manufacturers are diverging from withdrawal with
phenol and other natural solvents. This is for the reason that researchers do
not like to use or discard dangerous chemicals. Some manufacturers offer small RNA isolation kits that do not
contain organic solvents.
The micro spin column is a convenient tool
for maneuvering small volumes of affinity supports, usually between 5 µL and 100
µL, for protein purification. These columns enable you to purify more protein
in less time.
Some of the
beneficial features of these spin columns include:
·
They are perfect for an extremely small sample and
resin volumes.
·
They come with a column volume of 400 µL.
· They are available with different resin volumes,
ranging from 5 to 100 µL
The micro spin column is being used in a
variety of applications. In order to get the desired results, users have to add
the affinity sample and resin to the spin column. Then, they can use a
microcentrifuge to wash away impurities efficiently and elute their purified
sample without foregoing any resin during the process.
