Features of spin columns

Spin columns are largely used in molecular biology laboratories, as they allow the extraction of DNA commercially. They consist of a silica resin that binds DNA or RNA selectively according to the factors concerned with the extraction method. As a result, when you use a mini spin column to extract DNA or RNA, you will get only high-quality nucleic acid for cloning as well as long-range sequencing.

The purification of nucleic acids through a mini spin or a maxi spin column is considered a solid phase extraction technique. This is for the reason that these spin columns, whether they are mini or maxi, allow you to extract and purify nucleic acids easily and quickly. This extraction method mainly counts on the fact that DNA or RNA will bind to the solid silica phase under certain conditions, allowing you to extract nucleic acids of the highest quality.

Even before the nucleic acid methods used nowadays, it was known that in the existence of chaotropic agents, such as sodium perchlorate or sodium iodide, DNA binds to glass particles, silica, or to diatoms, which are unicellular algae, protect their cell walls with silica. This feature was employed to purify nucleic acids using silica beads or glass powder under alkaline conditions. Later, this was improved by making use of guanidinium hydrochloride or guanidinium thiocyanate as the chaotropic agent. Similarly, the use of glass beads was changed to silica gel.

Both the maxi spin columns, as well as mini spin columns were designed for safe and quick transfer. Nowadays, many suppliers offer outstanding quality of DNA spin columns, which allow users to purify nucleic acids rapidly in most biochemical and molecular applications. These spin columns are available in different sizes according to the extraction needs of users. Although a maxi spin or a mini spin column has the same capacity, they are available with different filters that are made of different materials, allowing you to use them in diverse biological applications.

These suppliers also offer dedicated extraction and purification kits to provide a quick spin-column-based technique to isolate sequencing-grade plasmid DNA. By making use of a single buffer, you will be capable of achieving bacterial cell resuspension, DNA binding, and lysis to purify nucleic acids within the minimum available time.

Some of the technical and salient features of a mini spin or a maxi spincolumn include:

·         They come with three different types of filters, such as Silicon, pulmonary embolus, and Glass.

·         Each of these filters comes with dissimilar bore sizes, ranging from two to eight layers.

·         A mini spin column is useful for different samples, including agarose gel or PCR products, E Coli, serum plasma, whole blood, and tissue.

Therefore, choosing the right filter will allow you to extract an assortment of DNAs, including DNA fragments, Plasmid DNA, Genomic DNA, and Viral RNA/DNA.

Above all, both spin Columns are so versatile, as they are designed from biocompatible polypropylene. They will also work well with all chromatography resins. They include a specific membrane that retains the resin as well as the sample in the column and prevents leak.

Tissue DNA isolation using the Mitochondrial DNA Isolation Kit

The process of Tissue DNA isolation usually starts with the lysis of tissues or cells in order to obliterate the protein structures and to allow the release of deoxyribonucleic acid or ribonucleic acid from the nucleus. It is vital to extract high-quality DNA from tissues and cells for several molecular biology applications and genomic applications, such as genotyping, screening, sequencing, and cloning.

Structurally, as eukaryotic cells and tissues are more intricate than microbial cells, there can be an assortment of technical obstacles to conquer. Fortunately, countless commercial kits as well as reagents are available, which are ready-to-use and they are specifically customized for isolating DNA from cells and tissues. One such kit is the mtDNA or Mitochondrial DNA Isolation Kit designed by LifeSpan BioSciences. The company is renowned for offering high-quality tissue arrays, antibodies, as well as immunohistochemistry information and services to researchers all over the world.

Mitochondria are semiautonomous organelles that work during apoptosis, aging process, anti-HIV drugs, as well as cancers. This tissue DNA isolation kit has a very high transformation rate and the changes in Mitochondrial DNA seem to be associated with certain diseases, like Alzheimer's disease, diabetes, and muscle disorders. Separation as well as quantification of Mitochondrial DNA is often necessary to study the relationships between the mtDNA and diseases.

The Mitochondrial DNA Extraction Kit offers convenient tools for separating high amounts and pure mtDNA from an assortment of tissues and cells, without contagions from genomic DNA. The disinfected DNA can be used for various studies such as Southern blotting, enzyme manipulations, cloning and PCR analysis as well as amplification.

The main purpose of Total RNA extraction is to purify the entire RNA extracted from various biological samples. Although there are many methods to extract RNA from the samples totally, the most commonly used method is guanidinium thiocyanate-phenol-chloroform. The extraction method involves lysing and eluting by making use of a filter paper that has a high throughput capability.

Although both Ethanol and Isopropanol are used to extract RNA from samples, Isopropanol is mostly used in the total RNA extraction process. This is for the reason that it allows precipitation of superior species and lower concentrations of RNA than ethanol, particularly if you keep it warm at low temperatures for a long time. This is also for the reason that RNA is less soluble in isopropanol, allowing it to precipitate quicker even at low concentrations.

Additionally, you will be capable of eluting large sample volumes by making use of Isopropanol. As less isopropanol is required for precipitation, you can often fit the solvent as well as your sample in one 15-ml tube. However, as salts are usually less soluble in isopropanol, they are inclined to co-precipitate with RNA. Therefore, isopropanol precipitation is the best option at room temperature with short brewing times, which will considerably minimize the probability of salt precipitation. Once you extract the RNA pellet from the isopropanol, you can clean it with cold ethanol to get rid of surplus salt. If you are certain that the sample does not hold a lot of salt, you can cool the isopropanol-precipitated sample.