A Micro Spin column is a convenient tool for maneuvering small amounts
of affinity supports, usually from 5 µL to 100 µL for protein distillation. For
products and other DNAs, ranging in volumes from 10 µL to 100 µL, Sephadex G-25
DNA Grade and spin-column chromatography are used for quick buffer desalting/buffer
exchange. Thus, these spin columns are excellent for speedy purification of
newly manufactured oligonucleotides more than 10-mers in 100 to 150 of
deprotection solution by making use of spin-column chromatography.
Micro Spin columns are celebrated
for their flexible features for experiments, allowing varying amounts of
Sephadex. They can be used for buffer exchange or desalting of DNA and elimination
of un-integrated radionucleotides, as well, from end-labeled oligonucleotides
in a capacity of 10 µL to 100 µL. This means that these spin columns can be
effectively used for any DNA more than 10 bases in length. Therefore, they are highly
appropriate for purifying oligonucleotides and fragmenting very small DNAs
following the mixture or a labeling reaction.
Some of the notable features of a
Micro Spin column include:
·
It is perfect for extremely small resin and example
volumes.
·
They can be effectively used with the column capacity
up to 400 µL and resin volume, ranging from 5 µL to 100 µL
·
They are the filter type columns that come with
a Polyethylene filter and with the pore size of about 30 µm
·
They are available with press-on bottom caps and
O-ring screw top caps
Users are just enough to add the
affinity sample and resin to one of these columns. They can then use a
microcentrifuge to remove contaminants efficiently and elute their cleansed sample
without losing any resin during the process. They also enable them to affinity cleanse
more protein in less time.
Micro Spin columns are used in a
variety of applications, including:
·
Affinity cleansing or chromatography
·
Immunoprecipitation
·
Immunodepletion
·
Co-immunoprecipitation
SYBR Green qPCR Mix mixes SYBR Green I dye, dNTPs, and AmpliTaq
Gold DNA Polymerase with dUTP, Passive Reference 1, and optimizes buffer in the
ease of solitary vial. The benefits of the mix include:
·
It reduces the assay setup time considerably
through the already combined components that are stored at 2 °C to 8°C.
·
No specific probes are required, as the dye in
the mix detects the double-stranded DNA efficiently.
·
It minimizes the formation of nonspecific
products, so superior performance can be achieved easily and quickly.
·
The dUTP in the mix decreases carryover pollution
considerably when used in combination with uracil-DNA glycosylase.
· The proprietary buffer developments of the mix ensure
reliability and performance.
The mix is also capable of
minimizing well-to-well unpredictability that can be caused by a variety of
reasons, such as sample evaporation and pipetting error. The dye in the mix is
perfect for target recognition or when a restricted number of assays are
required.
Above all, the SYBR Green qPCR Mix is celebrated for
its greatest flexibility and expediency at a reduced cost. This is for the
reason that it does not need any target-specific TaqMan probes for the process.
