Benefits of Micro Spin column and SYBR Green qPCR Mix

A Micro Spin column is a convenient tool for maneuvering small amounts of affinity supports, usually from 5 µL to 100 µL for protein distillation. For products and other DNAs, ranging in volumes from 10 µL to 100 µL, Sephadex G-25 DNA Grade and spin-column chromatography are used for quick buffer desalting/buffer exchange. Thus, these spin columns are excellent for speedy purification of newly manufactured oligonucleotides more than 10-mers in 100 to 150 of deprotection solution by making use of spin-column chromatography.

Micro Spin columns are celebrated for their flexible features for experiments, allowing varying amounts of Sephadex. They can be used for buffer exchange or desalting of DNA and elimination of un-integrated radionucleotides, as well, from end-labeled oligonucleotides in a capacity of 10 µL to 100 µL. This means that these spin columns can be effectively used for any DNA more than 10 bases in length. Therefore, they are highly appropriate for purifying oligonucleotides and fragmenting very small DNAs following the mixture or a labeling reaction.

Some of the notable features of a Micro Spin column include:

·         It is perfect for extremely small resin and example volumes.

·         They can be effectively used with the column capacity up to 400 µL and resin volume, ranging from 5 µL to 100 µL

·         They are the filter type columns that come with a Polyethylene filter and with the pore size of about 30 µm

·         They are available with press-on bottom caps and O-ring screw top caps

Users are just enough to add the affinity sample and resin to one of these columns. They can then use a microcentrifuge to remove contaminants efficiently and elute their cleansed sample without losing any resin during the process. They also enable them to affinity cleanse more protein in less time.

Micro Spin columns are used in a variety of applications, including:

·         Affinity cleansing or chromatography

·         Immunoprecipitation

·         Immunodepletion

·         Co-immunoprecipitation

SYBR Green qPCR Mix mixes SYBR Green I dye, dNTPs, and AmpliTaq Gold DNA Polymerase with dUTP, Passive Reference 1, and optimizes buffer in the ease of solitary vial. The benefits of the mix include:

·         It reduces the assay setup time considerably through the already combined components that are stored at 2 °C to 8°C.

·         No specific probes are required, as the dye in the mix detects the double-stranded DNA efficiently.

·         It minimizes the formation of nonspecific products, so superior performance can be achieved easily and quickly.

·         The dUTP in the mix decreases carryover pollution considerably when used in combination with uracil-DNA glycosylase.

·       The proprietary buffer developments of the mix ensure reliability and performance.

The mix is also capable of minimizing well-to-well unpredictability that can be caused by a variety of reasons, such as sample evaporation and pipetting error. The dye in the mix is perfect for target recognition or when a restricted number of assays are required.

Above all, the SYBR Green qPCR Mix is celebrated for its greatest flexibility and expediency at a reduced cost. This is for the reason that it does not need any target-specific TaqMan probes for the process.