Benefits of using ultra kits for tissue RNA/DNA isolation

Ribonucleic acid or RNA is a polymeric matter present in living cells as well as in many viruses. It consists of a long single-stranded chain of ribose and phosphate units with the nitrogen bases, such as adenine, cytosine, guanine, and uracil. These bases are tied to the ribose sugar. Ribonucleic acid is used in all the protein synthesis steps in all living cells and has genetic information for numerous viruses. High-quality tissue RNA isolation is a vital step necessary to do different molecular biology experiments.

Deoxyribonucleic acid or DNA is the inherited material present in humans as well as in all other organisms. Almost every cell in the body of an individual has the same DNA and it is located in the cell nucleus, which is known as nuclear DNA. However, a small amount of DNA can be found in the mitochondria, as well, which is often called mtDNA or mitochondrial DNA.    

                                                                       

Nowadays, many ultra kits are available for isolating DNA and RNA from tissues. These kits are designed for sequential tissue DNA isolation/RNA isolation from the same FFPE or formaldehyde-fixed paraffin-embedded or paraformaldehyde-fixed paraffin-embedded tissue sample. The RNA and DNA are recovered in individual eluates, and both will work well with a wide array of applications, including next-generation and real-time polymerase chain sequencing.

Some of the notable features of these tissue RNA isolation/ DNA isolation kits include:

·   They come with a flexible design, allowing the DNA/RNA isolation from tissues manually and automatically.

·       They need a negligible amount of samples for the isolation of DNA and RNA from tissues.

·     Some kits allow users to process as much as 40 µm of FFPE sections or curls using an alternative protocol.

·        These kits will work well with targeted DNA and RNA sequencing panels.

·      A consistent tissue DNA isolation, RNA isolation, or microRNA isolation can be achieved by using these kits, as they usually contain Dynabeads MyOne Silane.

The tissue DNA isolation and RNA isolation from the sample of the same FFPE make these kits a perfect method for preparing a sample for Oncomine Focus and Oncomine Comprehensive assays.

Releasing the information contained in FFPE samples is indispensable to cancer research. The isolation method of the common nucleic acid needs you to decide which nucleic acid to recover from a known sample. Alternatively, the RNA and DNA are purified jointly, or the sample is divided into half so that each of them can be cleaned separately.

Additionally, the kits address different issues by first separating DNA from the FFPE sample and afterward recovering the RNA from the DNA-exhausted supernatant. DNAs and RNAs are prepared as separate eluates and then, they are prepared for downstream analysis. This permits for a fuller investigation of valuable samples, including analysis of vital biomarkers, such as Copy number variants, hotspot mutations, indels, and gene fusions.

Another major benefit of using these kits for tissue RNA isolation/DNA isolation is that they allow for trouble-free scaling of the number of samples, which are processed either physically with a magnetic particle processor or with a magnetic stand.