Know the uses of a micro spin column

 

A micro spin column is a mini preparation spin column, which is mostly used for PCR products, plasmid, isolating genomic DNA, as well as for purifying the Agarose gel.DNA is selectively attached in a high-salt buffer to the membrane in these spin columns. In low-salt buffer or water, DNA is eluted after a wash step without desalting or alcohol precipitation.

A micro spin column is considered the most convenient tool for handling small capacities of affinity supports, usually from 5 µl to 100 µl, for purifying the proteins. Just adding the sample and the affinity to one of the micro spin columns and then using a microcentrifuge will make the process of washing away the impurities more efficient. It also aids greatly in eluting the purified sample without dropping any resin during the process.

A micro spin column is capable of allowing more proteins to purify the affinity in less time. These tools are ideal for extremely small resin and sample quantities. They are available in different column volumes as well as resin volumes.

Some of the beneficial features of micro spin columns include:

·         These spin columns play a vital role in extracting both Deoxyribonucleic acid and Ribonucleic acid efficiently, easily, and quickly.

·         As these columns are based on silica membrane, they will offer reliable results.

·         Micro spin columns are designed to work well with the Invitrogen’s as well as with Qiagen’s buffer.

·         They are available with a buffer recipe for PCR clean up and Gel extraction.

·         These spin columns are economical as well as suitable for large sample process, as well.

Above all, a micro spin column is capable of offering the highest quality of purified DNA. This makes these spin columns extensively used by many academic laboratories as well as in house applications.

An RNA and DNA Micro Spin column can also be employed for multiple applications, such as:

·         Affinity chromatography

·         Affinity purification

·         Immunoprecipitation

·         Immunodepletion

·         co-immunoprecipitation

They also play a crucial role in a variety of other applications, including:

·         Nano‐preparation of nucleic acids, viral, plasmid, and genomic DNA

·         To clean‐up DNA after enzyme reactions or PCR

·         Gel extraction

·         Concentration of RNA or DNA   

A Micro Spin column uses the same solutions used by a Mini Spin column, but it will use only one-third or one-sixth of the volume of each solution.   The highly developed design of these spin columns has eradicated problems related to buffer retention and dead volume, causing an increased recovery and purification of nucleic acids. The design aids users greatly in reducing the elution volume, as well, to as low as 5 μl.   

The major benefit of using a micro spin column is that it is capable of isolating the Deoxyribonucleic acid and Ribonucleic acid from a single colony or other tiny samples, such as a 96‐well microplate or saliva directly. 

Another notable benefit of using these spin columns is that there will be no dead volume and no buffer retention. A micro spin column is designed to be compatible with the DNA or RNA Kit Reagents of almost all manufacturers.