The protocols of tissue RNA and tissue DNA isolation

Isolation and purification of nucleic acids can be done effectively and quickly by making use of some dedicated kits, such as CST or Charge Switch Tissue kits. This means that you can use the Charge Switch gDNA Micro and Mini Tissue Kits for tissue DNA isolation. These kits allow fast and efficient sanitization of genomic DNA from samples. 

The beneficial feature of micro-tissue kits is that they will be capable of purifying up to 5 µg of genomic DNAs from 3 to 5 mg of tissue or 1to 2 mm diameter mouse ear clips. This sample size is appropriate for genomic DNA decontamination from micro-divided or laser capture micro-divided samples.

When considering the beneficial features of mini-tissue kits, they can purify up to 30 µg of genomic DNAs from 25 mg of tissue or 0.5 cm mouse tail tips. If you want to purify a spleen tissue, you can reduce the size of the tissue to 10 mg.

The gDNA Mini tissue kits need only 10 to 25 mg of tissue sample for purification. On the other hand, the gDNA Micro tissue kits will require only 3 to 5 mg quantities of tissue samples. Subsequent to the preparation of the lysates, you can purify DNA quickly, usually within 15 minutes by making use of ChargeSwitch Technology.

The ChargeSwitch Technology is a new magnetic bead-based expertise that offers a changeable surface charge reliant on the pH of the nearby buffer to facilitate the purification of nucleic acids. The CST beads in low pH conditions will have a positive charge that connects the negatively charged nucleic acid backbone. 

During the process of tissue DNA isolation or RNA isolation using these kits, proteins as well as other pollutants are not bound and are just swept away in an aqueous wash buffer. To isolate nucleic DNAs or RNAs, the charge on the bead surface is neutralized by increasing the value of the pH to 8.5 by making use of low salt removal buffer. Purified DNA or RNA will then elute right away into this removal buffer, thus, making the nucleic acids set for use in an assortment of downstream applications.

The ChargeSwitch Tissue Kits are intended to permit separation of genomic DNA as well as RNA from a variety of sources. The decontaminated genomic nucleic acid is appropriate to use in a range of downstream applications, such as polymerase chain reaction, Southern blotting, and restriction enzyme digestion.

Another benefit of using the Tissue RNA Isolation or DNA isolation kits is that they will allow you for isolating the genomic DNA from diverse types of animal tissues. The genomic nucleic acids are purified according to your preferences from other cellular proteinaceous parts. Usual yields of genomic DNA or RNA will vary according to the sample that is being processed.

Another major benefit of using ChargeSwitch Tissue Kits for the Tissue DNA Isolation or RNA isolation process is that they can be used without the need for dangerous chemicals, vacuum manifolds, or centrifugation. This is for the reason that these kits use the technology that is based on magnetic beads.