How does the mini spin column help to purify nucleic acids?

Nucleic acids can be purified using spin columns, as well. Purification of these acids, which is based on spin columns, is a solid stage extraction technique to purify them quickly. This method counts on the fact that nucleic acids will attach to the solid stage of silica under definite conditions.

Mini Spin Column has an all-in-one Silica Membrane, which has been extensively used as an economical substitute for Qiaquick, Qiaprep, Qiaamp, RNeasy, DNeasy, PureLink, PureYield, GeneElute, and more. In a separate single-column design, it is used in a variety of applications, ranging from Deoxyribonucleic acid to Ribonucleic acid, from normal PCR purification to the preparation of the next generation sequencing sample, and from plasmid to viral and genomic DNA.

Since the introduction of these spin columns, millions of them served scientists all over the world. Buffs have honored them in peer-evaluated publications with their individual mission-customized buffers for an assortment of applications. They are compatible with an extensive variety of buffers that carry different brand names.

The procedure involves different methods and stages to purify nucleic acids. The different stages include lyse, connect, clean, and elute. This means that lysis of cells, joining of nucleic acids to silica gel membrane, cleaning the nucleic acids connected to the silica gel membrane, and removal of the nucleic acids.

During the lyse stage, the cells of a sample are opened with a lysis method. This will break the cell membrane as well as the nucleus to discharge the nucleic acid.

During the binding stage, a buffer solution is added to the sample, together with isopropanol or ethanol to form the binding solution, which will then be transferred to the spin column. Then, the column is put in a centrifuge, which will force the binding solution through the inside silica gel membrane of the mini spin column. If the salt concentration and pH of the binding solution are most favorable, the nucleic acid will connect to the silica gel membrane while the solution traverses.

To clean, the flow-through is detached and a clean buffer is attached to the column. Again, the column is put in a centrifuge, forcing the clean buffer through the membrane. This will remove any residual contaminants from the membrane, leaving only the silica gel connected nucleic acid

During the elution stage, the wash buffer is taken away and water or an elution buffer is added to the spin column. Once again, the column is put in a centrifuge, forcing the removal buffer through the membrane, making the buffer to take the nucleic acid away from the membrane and it is collected from the column bottom.

Maxi spin column, which also includes a Silica membrane, is renowned for its extremely high binding capacity. This makes these spin columns run experiments with higher amounts of Deoxyribonucleic acid to acquire the curve completely to saturate. This binding will be higher than that of other columns.

Some of the other beneficial features of these spin columns include:

·         They can be used to extract both Deoxyribonucleic acids as well as Ribonucleic acids,

·         As the spin column comes with enough amounts of layers, it will offer a guaranteed result.

·         These columns are highly qualified for the purification of DNAs and RNAs.

·         The spin column is capable of dealing with maxi 50-ml samples.

Above all, the unique even-bottom-basket design of the maxi spin column ensures no liquid remains.